![]() ![]() It reproduces a little less low-bass, too, so you feel a touch less thump and rumble with bass-centric genres like EDM and hip-hop. Given its smaller size, this speaker doesn't get as loud as the PartyBox 310. Plus, the DJ and karaoke effects let you make the most of the included microphone and guitar inputs if you want to host a sing-a-long at your next party. Again, you get access to the JBL PartyBox app, where you'll find customization tools to switch up the colors and patterns for the RGB lights, as well as a graphic EQ and Bass Boost preset to change up its sound. It's a smaller and more affordable cousin to the JBL PartyBox 310, with many of the same fun features and tools to amp up the energy at your next big event. If you're shopping for a party speaker on a tighter budget, the JBL PartyBox 110 might be for you. ![]() Overall, this is a really powerful speaker that's lots of fun. ![]() You'll also find many sound customization tools in the app, so you can switch up the speaker's sound across the range using its graphic EQ or bump up the bass with the aptly-named Bass Boost tool. Karaoke lovers can plug their microphones and guitars right into the speaker to jam out with their favorite tunes, and the JBL PartyBox app brings some additional effects to adjust the microphone's performance for the best possible sound. Like many JBL party speakers, this device has some added tools to bring excitement to your next celebration. With a more than seventeen-hour battery life, you can keep dancing late into the night. Plus, it gets super loud, and its customizable RGB lights let you set the mood however you like. Right out of the box, this speaker brings lots of thump and rumble in the bass, meaning you can rattle the dance floor with genres like EDM and hip-hop. It's a great choice for your next big event, with many unique features designed to amp up the excitement as you rock out to your favorite tunes. The best speaker for parties we've tested is the JBL PartyBox 310. ![]()
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![]() ![]() ![]() ![]() To celebrate, I talked to the women (and one entrepreneurial 9-year-old girl) behind mother-daughter businesses. Today, it's a symbol of the long way women have come, and the exciting road ahead. In 1973, Congress designated the day to commemorate the August 1920 ratification of the 19 th Amendment granting women the right to vote. Read iDiva for the latest in Bollywood, fashion looks, beauty and lifestyle news.Women's Equality Day is on August 26. Lead image credit: Eros International + Vineyard Films Here's wishing a very happy Mother's Day to all! She breathes life into the film with her comic timing and range of emotions.Īs we continue our battle against COVID-19, let's take a moment to celebrate and express our love for our pillars of strength, our refuge in this storm-our mothers! This Mother's Day, iDiva's writers pen special tributes to their mothers and the beautifully complex relationship that exists between a mother and her daughter. SMS worked to a large extent solely because of her character-the nosy yet always interested mother, Vimla. Shubh Mangal SaavdhanĪll hail Seema Pahwa and her effortless onscreen presence. The movie is brilliantly cast, excellently executed and highly recommended for those who want to end up sobbing uncontrollably.Ĭredit: JAR Productions 7. Set in rural India, the story follows Apeksha, a class 10 student who unintentionally inspires her single mother, Chanda to complete her education as well. Swara Bhasker has always delivered and this movie is an excellent example of that. ![]() We highly recommend!Ĭredit: Alchemy Productions 6. It shows the complex rollercoaster of emotions that is a part of every mother-daughter relationship and highlights just how much has changed with the generations. Tribhanga starring Kajol, Mithila Palkar and Renuka Shahane is the real, REAL mother-daughter relationship movie that India needed. Plus, anything Sheeba Chaddha touches, it turns to gold like this quirky film. One can easily understand the transparency that existed between the two and that’s something that rarely finds onscreen representation. The scenes with Renee and her mom might have been short but that was enough for us to fall in love with them. The fact that mothers are willing to be this selfless yet so encouraging is why we don’t deserve mothers.Ĭredit: Aamir Khan Productions 4. Secret SuperstarĪlthough the movie was centered around Insia’s career as a budding singer, the simultaneous storyline of her mom trying to protect her at all costs from her violent father is a tearful side plot that definitely deserves attention. From dealing with school teachers and authorities to figuring out what’s best for your kid, the show is quite informative about the stresses of a modern mom.Ĭredit: Film Farm India 3. Karisma Kapoor’s digital debut with ALTBalaji, Mentalhood, showcases the hectic life of a new-age 21st century mother. From discussing financial independence to deciding the right time to move out of home, Masaba Masaba is a guidebook for all the young, fiercely-motivated and slightly-confused women out there and their mothers. Starring real-life mother-daughter duo, Neena and Masaba Gupta, the show is an excellent depiction of modern day relationships. ![]() ![]() ![]() The Kinsey Institute is also grateful for a financial gift from Desert Harvest to support the processing and digitization of the Cynthia Plaster Caster Collection. Knowing that we are charged with preserving her legacy for decades to come is such an honor to all of us at the Institute and to me personally.” “I knew about Cynthia from the time I was a teenager - I went to art school in Chicago and she was already an incredibly well-respected and adored artist and music lover. “We are so thrilled to be the permanent home of Cynthia’s life’s work,” said Rebecca Fasman, Kinsey Institute Curator. Her notebooks include entries about the locations she visited, and her observations, sketches, and portraits of some of the musicians and people she met. Cynthia preserved her tickets to music shows from the 1960s and the 1970s with legendary performers like the Beatles, the Kinks, Frank Zappa and the Mothers of Invention, and Jimi Hendrix. The Plaster Caster collection includes Cynthia’s “Plaster Casters of Chicago” suitcase – which served as both portable storage for her casting tools and as a highly-visible calling card that she used to attract the attention of musicians and their entourages - as well as a host of additional music memorabilia that paints a picture of the vibrant early world of rockers and groupies. This is about contextualizing things so her archives can be looked at and researched in the future by scholars." That’s something we were passionate about. “They have an archive that is accessible to people. “The Kinsey Institute is a teaching institution,” Kellner added. “We know the Kinsey Institute can provide the attention and sincere representation of Cynthia’s vast talent and life mission.” “Cynthia wanted people to know who she was and what her role in music history was, rock and roll musical history, especially,” Novak said. “She did it in a very unorthodox and original way - how cool is that? No one else had the bravery to memorialize someone this way.”įollowing her death in April 2022, long-time friends Babette Novak and Chris Kellner contacted the Kinsey Institute regarding her collection. “What I see in Cynthia’s work is twofold: this amazing respect she had for the musicians and the way that she honors them with her art and her craft,” said Melanie Cooper Pennington, a lecturer in sculpture in the IU Eskenazi School of Art, Architecture + Design and affiliate faculty of the Kinsey Institute. Her many diaries include meticulous notes she took of each casting session and document her commitment to continually evolving and improving the composition of her casting materials and her molding technique. Additional items in the collection offer a fascinating glimpse of Cynthia’s artistic process. The new collection at the Kinsey Institute includes many of these famous plaster penis molds, bronze penis casts, breast casts, and a plethora of original drawings, prints, notes, and annotated books. In 2000, she began casting female musicians’ breasts including Suzi Gardner of L7, singer Peaches, and Karen O. Many of these plaster molds were also cast in bronze. From 1968 through 2014, she cast over 70 phalluses of mostly rock and punk musicians, tour and road managers, actors, and artists, including Jimi Hendrix, Jello Biafra of the Dead Kennedys, Pete Shelley of the Buzzcocks, actor and singer Anthony Newley, and “Ivan” of the Flying Karamazov Brothers. The Kinsey Institute at Indiana University has acquired an historic collection of artwork, memorabilia and personal effects from renowned artist Cynthia Albritton, also known as Cynthia Plaster Caster.Ĭynthia Plaster Caster became famous for creating plaster casts of musicians’ erect penises. ![]() ![]() In Bend, pinball machines are more common than you'd think. There are a couple of pinball museums in the country, the most significant being down at the Las Vegas Pinball Hall of Fame where 400 or so machines blink inside a massive showroom. In fact, they say the place is seeing a steady increase in patronage, complete with (as of this printing) 26 machines, all well maintained and all functioning properly. It's not just a nerd-centric audience flocking to pinball at the Ground Kontrol, as their management tells me. In Portland, there's a place called Ground Kontrol that boasts the largest collection of pinball machines in the Northwest. For example, last weekend a pinball convention brought some 350 different machines, and a few thousand people eager to play them, to the Seattle Center. Pinball has become increasingly popular, especially here in the Northwest. At least I haven't for the past decade or so, but I'm a sucker for pinball - when the opportunity arises. Nevertheless, I drop another three quarters in because obsession is a real son of a bitch. ![]() And once again, I see the ball rolling down the middle of the table, splitting the difference between the two flippers and creating an all-too-familiar clunk as it lands in the mechanical mystery zone that is the inside of a pinball machine. I think - as I've thought at other places during my week of intense pinball immersion - that I've got this thing figured out. This is my fifth pinball stop of the day and even as my wrists become a bit sore and my pint glass again empty, I can't find a good stopping point. I've invested a couple dollars into this device, which happens to be promoting the popular television program, CSI (which I've never seen), and I feel a few more bucks worth of quarters burning a hole in my pocket. So it doesn't make a whole hell of a lot of sense that I'm in the back of a dark bar swearing at a pinball machine. ![]() ![]() They're drinking on patios and wearing bright colors and stylish sunglasses. It's a perfectly nice day outside, one of the first in a long while. ![]() ![]() ![]() ![]() SMD stuff is too modern for it though so I then have to fall back to internet searches. The internet has changed searching for equivalents but I often find the Towers book faster as I grew up with it and know many favourite equivalent transistors. It lists the transistors specifications and any equivalent (but not all) You can flick through various ranges of transistors and select a suitably specified part. I still have my copy from the 1980's and it still serves me well. In the UK when I worked as a 'Saturday lad' in an electronics shop, I used the 'Towers Transistor selector' book. Substitution of RF power devices needs to be approached with a great deal of caution,& preferably with a Spectrum Analyser on hand. Modern devices as a rule,are better spec'd than older ones,so a common modern type may replace an old special type. In more complex circuits,have a look at what devices other manufacturers use in similar circuits-there may be a common device which will do the job. If it is something simple,like a lamp,LED,or relay driver,you can probably replace it with a more common type without any drama. If you can't find a listed equivalent,one good philosophy is to look at the circuit the device is used in. Not only did they often disagree,but by the time you had tracked from one book to another,the specs on the final one you turned up were many times quite different to the original. At one of my earlier jobs,we had semiconductor equivalents book from several sources. Good luck finding anything that will do that!(though it must be out there somewhere) Many times the equivalents shown in the various books are harder to find than the original component. ![]() ![]() Enjoy $5 green beer (while supplies last), $6 Guinness, $8 Jameson shots, and $8 Irish Drop shots! Admission is free. Patrick’s Day specials all day long on Friday, March 17, 2023. This event will feature live music from the Trevor Clark Band, drink specials, and March Madness games on the TVs all day long! Guests can also expect food specials including $5 Dublin Nachos, $5 Corned Beef Sliders, and a $14 combo of Shepard’s Pie + a pint. The first Fat Bottom Friday will take place on St. Expect green beers, dancers, leprechauns, DJs on the rooftop, live bands on the main stage, and drink specials all day long, including green beer and Proper 12 Whiskey drinks. Patrick’s Day Party is Friday, March 17, 2023, at Famous Nashville from 10:00am to 3:00am. GURU DISCOUNT: Use promo code GURU to get $5 OFF tickets! Redeem discount! Tickets are $20 in advance and $25 day-of. Guests can expect DJ Guy Fell in the beer garden, steins of green beer, drink specials, food specials, and live music. Von Patrick’s Day is Friday, March 17, 2023, from 11:00am to 8:00pm at Von Elrod’s. GURU DISCOUNT: Use promo code GURU to get $2 OFF tickets! Redeem discount! Tickets are $15-$20 plus fees in advance. Attendees can expect a day-long festival featuring nationally recognized artists across multiple stages, drink specials, numerous bars, and more. Patrick’s Day block party, is Friday, March 17, 2023, from 4:00pm to midnight at Tin Roof on Demonbreun. The 13th annual Lucky Stache Bash, Nashville’s biggest and longest-running St. Patrick’s Day PARTIES AND EVENTS IN NASHVILLE Paddy’s Celebration = Don’t Get Pinched + Drink Green Beer St. ![]() ![]() Consider this your go-to resource for all things St. Paddy’s Day falls during most people’s spring break, so these are some fun ways to enjoy Nashville while soaking up the luck of the Irish. Patrick’s Day is officially on Friday, March 17, 2023. ![]() ![]() ![]() The Board of Veterans’ Appeals (also known as “BVA” or “the Board”) is a part of the VA, located in Washington, D.C. Notice of an appeal must be received by the court with a postmark that is within 120 days. Court of Appeals for Veterans Claims, an independent court that is not part of the Department of Veterans Affairs. Appellants may present their cases in person to a member of the Board at a hearing in Washington, D.C., at a VA regional office, or by videoconference.Ī final Board of Veterans’ Appeals decision that does not grant a claimant the benefits desired may be appealed to the U.S. Although it is not required, a Veterans service organization, an agent, or an attorney may represent a claimant. ![]() The Board of Veterans’ Appeals makes decisions on appeals on behalf of the Secretary of Veterans Affairs. Go to and scroll down to the PDF on the “Veterans Appeals Process Briefing” for specific details on the appeals process.īOARD OF VETERANS’ APPEALS WHAT IS THE BOARD OF VETERANS’ APPEALS? There are 98 BVA judges and 67,000 Veterans waiting for hearings. The Hearing Docket is based on availability but typically takes over 365 days. The BVA judge will make a decision based on the hearing and the evidence submitted. Following the hearing, the claimant will have 90 days to submit new evidence. The claimant will be placed on a docket (waiting list) for a hearing before a judge by videoconference (or in DC). Hearing Docket – in this appeal, the claimant is requesting a hearing before a BVA judge. The Evidence Docket appeal will be adjudicated on average over 365 days. The BVA judge will consider the new evidence provided. The claimant has 90 days from the NOD to submit new evidence. The Direct Docket appeal will be adjudicated on average up to 365 days.Įvidence Docket – in this appeal, the claimant has new evidence for the BVA judge to consider. No new evidence may be submitted, and the BVA judge will review the same record and make a decision. The Higher Level Review goal is to be adjudicated within 125 days.Īppeal to Board of Veterans Appeals (BVA) (Notice of Disagreement – NOD) The appellate has three options under AMA with an appeal to the BVA:ĭirect Docket – in this appeal, the appellate believes that a mistake was made. HLR is a request for a review by a senior adjudicator based on a belief that a mistake was made, and no new evidence may be submitted. Higher Level Review (HLR) – this process does not allow the claimant to submit additional evidence and is based solely on the evidence already of record. The Supplemental Claim goal is to be adjudicated within 125 days. ![]() ![]() With the SC, there is a “duty-to-assist” and the VA will assist in the location of evidence in support of a claim. Supplemental Claim (SC) – this process allows the claimant to submit “new and relevant” evidence in support of the claimed issue. Under the AMA, there are three distinct appellate review processes: Typical issues appealed are disability compensation, pension, education benefits, recovery of overpayments, reimbursement for unauthorized medical services, and denial of burial and memorial benefits.Ī claimant has a Limiting Date of 1-year from the date of Notification of a VA decision to pursue a course of action under the Appeals Modernization Act of 2017 (AMA). The AMA was signed into law and took effect on 19 February 2019. Veterans and other claimants for VA benefits have the right to appeal decisions made by a VA regional office, medical center, or National Cemetery Administration (NCA) office. ![]() ![]() ![]() Gold sheen obsidian allows you to reclaim your personal power and follow a life path that will ultimately balance you and reveal your hidden talents/skills. ![]() It helps protect you from negativity and release egotistical thoughts or blockages preventing your spiritual growth. 1 Full Strand 15' Genuine Natural Black Obsidian Round Feng Shui Beads Good Fortune Protection Healing Gemstone Beads 6mm 8mm 10mm 12mm 14mm (19k) 5.69. Leavy notes these varieties usually come from countries in Central America, like Mexico or Guatemala, where there's a lot of volcanic activity. But there's also silver sheen and gold sheen obsidian, which have a silver or gold tint, respectively. As we already know, it is a volcanic glass, just like black obsidian, except it features golden sparks.Obsidian belongs to the silicate group of minerals with a hardness scale of 5 to 5.5, with some varieties being harder than others. This is a great stone to use during introspective meditation and to better understand yourself. In most crystal shops, you'll find black obsidian, which is a rich, opaque black color. Physical Properties Of Gold Sheen Obsidian. ![]() Gold sheen obsidian is known for allowing people to gain insight on the root of their problems and discover where true healing needs to happen. These varieties commonly come from nations in Central America, like Mexico or Guatemala, with numerous volcanic activities. But there’s also silver sheen and gold sheen obsidian, that have a silver or gold tint, respectively. Though not considered a gemstore, its natural properties still are valued in the spiritual community because of its ability to help people progress their personal growth. In most crystal stores, you may find black Obsidian, a rich, opaque black color. Obsidian naturally forms as volcanic glass after lava with high silica content cools down rapidly. PERSONAL POWER & LIFE PURPOSE Stunning Goldsheen Obsidian heart - powerful grounding energy Use Gold Sheen Obsidian to reclaim your personal power and. Gold Sheen Obsidian is a type of obsidian that typically looks brown with a golden sheen. Arfvedsonite Arfvedsonite is a rare mineral found in igneous rocks and can be identified by its dark green or black prismatic crystals. GREAT FOR: Divination, meditation, introspection, personal growth, spiritual growth, insight, protection, release negative energy ![]() ![]() We all love the food at Disneyland and Walt Disney World, but it’s usually not something we can take with us. Once again, check out all of my thoughts in our Best Food at Disneyland for Disney100 guide, and be sure to let me know below what your favorites are! The Best Disney100 Merchandise That Every Disney Fan Needs to Look Out For I’d also highly recommend the Potato & Cheddar Cheeseburger at the Hungry Bear Restaurant as it has the most perfect crunch with every bite that is made even better by its gooey but not too messy bacon cheddar sauce and green chilies. The soda is very refreshing and it even comes with a sour strip that adds a burst of flavor whenever you need it. You may think it would be too much sweetness and sugar, but they actually balance each other out nicely. I also recommend pairing the pretzel with a Sparkling Grape soda, which is also available at the Refreshment Corner. It honestly got better with every bite, and it’s making me sad thinking about it because I can’t have one right now. It was a deliciously soft pretzel stuffed with a wonderful cream cheese frosting, and it was topped with a generous drizzle of salted vanilla cream and lavender sugar. Hands down, my favorite food item at Disneyland for the Disney100 celebration was the Violet Pretzel at the Refreshment Corner. You can check out my full thoughts and comments from one of the creators behind the menu in our Best Food at Disneyland for Disney100 guide and in the slideshow below, but I wanted to share a few thoughts with you below for some of my favorites. It’s also a joy each time you go to the park as there is usually some festival or special event that brings with them new options you’ve never tried before, and Disney100 is no different.įor Disney100, Disney has created a menu filled with items that honor the company’s past and aim to tell a story with each bite, and I had the chance to try all but two of them. One of the best things to do at any Disney park is try all the wonderful and delicious food that you can’t get anywhere else. ![]() Disney100 In Summary: A Party to Kick Off a New Centuryĭisney100 Food, From the Incredible Violet Pretzel to a Delicious Potato & Cheddar Cheeseburger.The Disney 100 Years of Wonder Exhibit Is a Walk Down Memory Lane Featuring Disney’s Biggest Attractions and Moments. ![]() Disneyland’s Version of Mickey & Minnie’s Runaway Railway and Toontown’s Reopening.World of Color - ONE: The First Disneyland Spectacular to Feature Disney, Pixar, Star Wars, and Avengers.Wondrous Journeys: A Heartfelt Celebration of Every Disney Animation Studios Movie.The Best Disney100 Merchandise That Every Disney Fan Needs to Look Out For.Disney100 Food, From the Incredible Violet Pretzel to a Delicious Potato & Cheddar Cheeseburger.I encourage you to watch the videos, click through the slideshows, and share in the comments how you’ll be celebrating Disney100! Additionally, you can read through the entire guide, or click the links below to jump right to the section you’d like to see… ![]() ![]() ![]() ![]() Heat the samples and 95 degrees C for five to 10 minutes in a sample buffer containing a reducing agent such as beta-mercaptoethanol. The proteins are then transferred onto a membrane where they can be detected using antibodies. The purpose of western blotting is to separate proteins on a gel according to the molecular weight. Protocols are provided by Abcam “AS-IS” based on experimentation in Abcam’s labs using Abcam’s reagents and products your results from using protocols outside of these conditions may vary. Watch our easy-to-follow video protocols.View AbExcel secondary antibodies for exceptional western blots.View our list of available positive control lysates, blocking peptides, and positive control proteins.Lysates can be aliquoted and stored at -20☌ for future use.Īll lanes: beta Actin antibody - loading control (ab8227) at 1/5000 dilution To reduce and denature your samples, boil each cell lysate in sample buffer at 100☌ for 5 min.We recommend reducing and denaturing the samples using the following method unless the online antibody datasheet indicates that non-reducing and non-denaturing conditions should be used. Determine how much protein to load and add an equal volume 2X Laemmli sample buffer..Determine the protein concentration for each cell lysate. Remove a small volume of lysate to perform a protein quantification assay.Gently remove the tubes from the centrifuge and place on ice, aspirate the supernatant, and place in a fresh tube kept on ice discard the pellet. Centrifuge for 20 min at 12,000 rpm at 4☌ in a microcentrifuge.The minimum concentration is 0.1 mg/mL, optimal concentration is 1–5 mg/mL. Volumes of lysis buffer must be determined in relation to the amount of tissue present protein extract should not be too dilute to avoid loss of protein and large volumes of samples to be loaded onto gels. For a ~5 mg piece of tissue, add ~300 μL of ice-cold lysis buffer rapidly to the tube, homogenize with an electric homogenizer, rinse the blade twice with another 2 x 200 μL lysis buffer, then maintain constant agitation for 2 h at 4☌ (eg place on an orbital shaker in the fridge). Store samples at -80☌ for later use or keep on ice for immediate homogenization. Place the tissue in round-bottom microcentrifuge tubes or Eppendorf tubes and immerse in liquid nitrogen to snap freeze.Dissect the tissue of interest with clean tools, on ice preferably, and as quickly as possible to prevent degradation by proteases.Gently remove the tubes from the centrifuge and place on ice, aspirate the supernatant and place in a fresh tube kept on ice, and discard the pellet.You may have to vary the centrifugation force and time depending on the cell type a guideline is 20 min at 12,000 rpm but this must be determined for your experiment (leukocytes need very light centrifugation). Centrifuge in a microcentrifuge at 4☌.Maintain constant agitation for 30 min at 4☌.Alternatively, cells can be trypsinized and washed with PBS prior to resuspension in lysis buffer in a microcentrifuge tube. Scrape adherent cells off the dish using a cold plastic cell scraper, then gently transfer the cell suspension into a pre-cooled microcentrifuge tube.Aspirate the PBS, then add ice-cold lysis buffer (1 mL per 10 7 cells/100 mm dish/150 cm 2 flask 0.5 mL per 5x10 6 cells/60 mm dish/75 cm 2 flask).Place the cell culture dish on ice and wash the cells with ice-cold PBS.View our western blot protocol video below. Solutions and reagents: running, transfer, and blocking buffers.The membrane can then be further processed with antibodies specific for the target of interest and visualized using secondary antibodies and detection reagents. The gel is placed next to the membrane and the application of an electrical current induces the proteins to migrate from the gel to the membrane. The immunoassay uses a membrane made of nitrocellulose or PVDF (polyvinylidene fluoride). Western blotting is a technique that uses specific antibodies to identify proteins that have been separated based on size by gel electrophoresis. Our western blot protocol includes solutions and reagents, procedures, and useful links to guide you through your experiment. ![]() |
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